Graduation Semester and Year
2018
Language
English
Document Type
Thesis
Degree Name
Master of Science in Materials Science and Engineering
Department
Materials Science and Engineering
First Advisor
Kyungsuk Yum
Second Advisor
Fred Harry Tibbals
Abstract
This thesis focuses on the characterization of bone with the help of Fourier Transform Infrared Spectroscopy and Energy Dispersive Spectroscopy. Such analysis of the physics and chemistry of biomaterials is vital to resolve problems in life science of bone-related diseases and disorders. Material characterization can help in the understanding of disease mechanisms and lead to useful drugs and other treatments. In this thesis, I have used bone materials produced by groundbreaking research at UT Southwestern Research Center and Texas Scottish Rite Children's Hospital to establish that idiopathic clubfoot (Talipes equinovarus) is associated with the Follistatin 5 gene. We studied healthy wild-type laboratory rats in comparison with genetically modified rats, called knock-out type, in which function of the Follistatin 5 gene was controlled with genetic engineering. We were able to identify significant differences in mineral and matrix composition of bone despite considerable variability in the samples. For mid-diaphysis of bone, matrix content was reduced in the knockout compared to the wild-type, leading to the increased mineral to matrix ratio in the knockout.
Keywords
Bone, FTIR
Disciplines
Engineering | Materials Science and Engineering
License
This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 International License.
Recommended Citation
Yadav, Sunil Balasaheb, "QUANTITATIVE ANALYSIS OF MINERAL/MATRIX TO EVALUATE GENETICALLY ALTERED BONE WITH INFRARED SPECTROSCOPY AND X-RAY SPECTRAL IMAGING" (2018). Material Science and Engineering Theses. 138.
https://mavmatrix.uta.edu/materialscieng_theses/138
Comments
Degree granted by The University of Texas at Arlington