Document Type

Honors Thesis

Abstract

Groundwater is relied on for drinking by more than 50 percent of the United States. For this reason, the purity of groundwater is extremely important. There has been possible contamination of opportunistic pathogenic microorganisms in groundwater attributable to oil and gas extractions from drilling and hydraulic fracturing; two of which are Pseudomonas aeruginosa and Bacillus cereus. These pathogens can cause severe infections in humans. P. aeruginosa increases prevalence of chronic and nosocomial infections. B. cereus produces toxins that are one of the most common causes of food poisoning. B. cereus can also cause infections of the eye, respiratory tract, and in wounds. Here, we quantitatively analyze possible metabolic therapies for these microorganisms by developing a metabolic profile for P. aeruginosa and B. cereus combining known secreted or absorbed metabolites for these microbes with common metabolites found in nutrient and potato dextrose broth, through the use of liquid chromatography- mass spectrometry.

Publication Date

5-1-2020

Language

English

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