Electrical detection of single-base DNA mutation using functionalized nanoparticles

Authors

Samir M. Iqbal
Shawn Christensen
Swati Goyal
Mohammud R. Noor

Document Type

Article

Source Publication Title

Virtual Journal of Nanoscale Science & Technology

DOI

http://dx.doi.org/10.1063/1.3152768

Abstract

We report an electrical scheme to detect specific DNA. Engineered hairpin probe DNA are immobilized on a silicon chip between gold nanoelectrodes. Hybridization of target DNA to the hairpin melts the stem nucleotides. Gold nanoparticle-conjugated universal reporter sequence detects the open hairpins by annealing to the exposed stem nucleotides. The gold nanoparticles increase charge conduction between the electrodes. Specifically, we report on a hairpin probe designed to detect a medically relevant mutant form of the K-ras oncogene. Direct current measurements show three orders of magnitude increase in conductivity for as low as 2 fmol of target molecules

Disciplines

Electrical and Computer Engineering | Engineering

Publication Date

8-17-2009

Language

English

License

This work is licensed under a Creative Commons Attribution-Share Alike 4.0 International License.

Recommended Citation

Iqbal, Samir M.; Christensen, Shawn; Goyal, Swati; and Noor, Mohammud R., "Electrical detection of single-base DNA mutation using functionalized nanoparticles" (2009). Electrical Engineering Faculty Publications. 27.
https://mavmatrix.uta.edu/electricaleng_facpubs/27