PREPARATION OF DUAL FLUORESCENCE AGENTS-LOADED NANOPARTICLE AND ITS IN VITRO AND IN VIVO APPLICATION
Graduation Semester and Year
2018
Language
English
Document Type
Thesis
Degree Name
Master of Science in Biomedical Engineering
Department
Bioengineering
First Advisor
Liping Tang
Second Advisor
Yi Hong
Third Advisor
He Dong
Abstract
There’re many existing imaging tools that composed of different imaging moieties to improve both resolution and sensitivity, however there’s not yet any imaging method that could be effectively utilized in both in vitro and in vivo study. To fill the gap, dual dyes-labeling fluorescent imaging nanoprobes was synthesized to simultaneous carry both visible fluorescent isothiocyanate (FITC) dye and near infra red (NIR) dye, 1,1′,3,3,3′,3′- hexamethylindotricarbocyanine iodide dye agent (HITC). For that, nanoparticles (NPs) was synthesized from 2-Hydroxyethyl methacrylate, styrene, acrylic acid and fluorescein-o-acrylate which contains visible FITC dye. The NPs were then labeled with HITC dye via physical entrapment to procedure HITC-FITC NPs. These nanoparticles have average size of 142.9nm±2.2, with high HITC loading efficiency (>90%). Our results confirm that both FITC and HITC dyes were successfully incorporated into NPs with high fluorescent intensity in both visible and NIR ranges. We have also observed possible static quenching effect caused by high concentrations of both dyes, whereas concentration of HITC increases reduce fluorescent intensity of FITC in NPs. By varying dye concentrations and ratios, we are able to fabricate HICT-FITC NPs with significant intensities in both NIR and visible ranges, with HITC/NPs weight ratio of 4X 10-4. Our results also confirmed that HICT-FITC NPs exhibits high photostability and colloid stability, as well as high aqueous-stability overtime. These particles also have low cytotoxicity with cancer cells, with upper limit concentration of 0.3mg/ml. With this particle concentration, different types of cells, esophageal, prostate, fibroblast and macrophage cell, were labeled and high resolution cell images was able to produce by microscope at visible light channel. Finally, particles labeled cancer cells was able to be monitored in animal model under near infra-red range imaging
Keywords
Nanoparticle, Near infrared dye, Fluorescein isothiocyanate, Styrene
Disciplines
Biomedical Engineering and Bioengineering | Engineering
License
This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 International License.
Recommended Citation
Vu, Hong-Hao Thi, "PREPARATION OF DUAL FLUORESCENCE AGENTS-LOADED NANOPARTICLE AND ITS IN VITRO AND IN VIVO APPLICATION" (2018). Bioengineering Theses. 206.
https://mavmatrix.uta.edu/bioengineering_theses/206
Comments
Degree granted by The University of Texas at Arlington