Graduation Semester and Year

Summer 2025

Language

English

Document Type

Dissertation

Degree Name

Doctor of Philosophy in Mechanical Engineering

Department

Mechanical and Aerospace Engineering

First Advisor

Hyejin Moon

Abstract

This dissertation introduces intelligent microfluidic platforms by combining advanced DEP-based manipulation with real-time visual feedback. A DEP device featuring circular corral traps and dual-plane electrodes enables precise submicron particle trapping, high-resolution particle separation, and cell-particle co-assembly. Simulations and experiments confirm enhanced electric field control and stable confinement. To enable adaptive operation in EWOD systems, a deep learning model (U-Net) was developed for real-time droplet meniscus segmentation. The model achieved 98% accuracy and remained robust under noisy, low-contrast conditions. A live video pipeline was implemented, enabling consistent frame-by-frame feedback for closed-loop control. Together, these innovations establish a foundation for autonomous, high-performance microfluidic systems in biomedical, diagnostic, and manufacturing applications.

Keywords

Dielectrophoresis, Machine Learning, Droplet Segmentation, Intelligent Microfludic Systems

Disciplines

Electro-Mechanical Systems

License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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