Graduation Semester and Year
2016
Language
English
Document Type
Thesis
Degree Name
Master of Science in Materials Science and Engineering
Department
Materials Science and Engineering
First Advisor
Pranesh B Aswath
Abstract
The use of bioactive material from bioceramic and composite components to support bone cell and tissue growth is an interesting area of medical field. Calcium phosphate ceramics are biocompatible and may develop interactions with human living bone tissues. Bioactive calcium phosphates, beta -tricalcium phosphate (β-TCP), have been intensively investigated as the cell scaffold for bone tissue engineering because it is well recognized that they are compatible to natural bone tissue and osteoconductive. In this research we will discuss two types of biomaterial with different kind of composition—(a) a cement type and (b)a scaffold type of biomaterial that can be applied to different kind of bone effect treatment. β-TCP is advantageous from the viewpoint of dissolution, but brittle compare to the other CPC. Its brittleness posses a limitation of their common clinical use. Current challenges include the engineering of materials that can match both the mechanical and biological property to enhance the recovery of bone failure. For the study, bioactive glass, gelatin, and laponite solution which that are is known forto their bioactive propertiesfeature will be usedapplied to create a bioactive scaffold with β-TCP. Groups of scaffold will be analysis in three direction approaches (1) Material characterization by using SEM, FTIR, XRD, EDS, goniometric machine…etc (2) In Vitro Study with cell culture, MTS assay, cell adhesion, raman spectra….etc (3) Development of TThree Dimensional Printed Scaffold. Findings suggest that better the optimum composition of b-TCP with 30% bioglass and eitherwith 2wt% laponite orand 2wt% Gelatin can lead to higher mechanical property and more enhanced formation of hydroxyapatite(HA). Cellular differentiation studies with xxxx cells in the presence involved addition of ascorbic acid (50 mg/L) also well performed demonstrated improved by observing the collagen fiber bundles formation compared to other compositions and the control coverslip. These results support the larger concept that beta tri-calcium phosphate with Bioglass and great binder like gelatin and laponite could control bone formation by inducing the osteogenic differentiation. With the understanding of the material characterization and pathology environment, using specialized three-dimensional (3D) printing technology can we created a porous structure to promote bone repair.
Keywords
Beta tri-calcium, Biomaterials, CPC, Biocement, Bioscaffold, Gelatin, Laponite
Disciplines
Engineering | Materials Science and Engineering
License
This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 International License.
Recommended Citation
Yao, Chien-Ning, "OSTEOGENIC DIFFERENTIATION IN PREOSTEOBLAST MC3T3-E1 CELLS INDUCED BY BETA TRICALCIUM PHOSPHATE BIOGLASS COMPOSITE" (2016). Material Science and Engineering Theses. 111.
https://mavmatrix.uta.edu/materialscieng_theses/111
Comments
Degree granted by The University of Texas at Arlington