Graduation Semester and Year
Spring 2024
Language
English
Document Type
Thesis
Degree Name
Master of Science in Materials Science and Engineering
Department
Materials Science and Engineering
First Advisor
Dr. Seong Jin Koh
Second Advisor
Dr. Kyung Suk Yum
Third Advisor
Dr. Ye Cao
Abstract
A rapid, portable, and highly sensitive method for detecting specific pathogens like Covid-19 would be invaluable for public health and emergency responses. The current standards like PCR and RT-PCR, are incredibly sensitive but also time-consuming, require skilled personnel, and demand substantial lab space. Here, we propose an alternative method capable of detecting specific oligonucleotide sequences within just 3 minutes, using a portable silicon or glass substrate (6 mm x 9 mm). We demonstrated this using 67-mer oligonucleotides with a Covid-19 sequence as a model system, achieving a detection sensitivity down to 1 nM. Our approach involves sandwiching target COVID-19 oligonucleotides between a 50 nm capture Au nanoparticle (C-AuNP) and 30 nm probe Au nanoparticles (P-AuNPs), creating a core-satellite nanostructure on the substrate. Detection occurs through the red shift of the plasmon resonance peak compared to a control without Covid-19 oligonucleotides. This method allows for the rapid detection of COVID-19 oligonucleotides within a total hybridization time of just 3 minutes. Moreover, the degree of red-shifted plasmon resonance peak absorbance difference correlates with the concentration of target oligonucleotides, enabling quantitative detection
Keywords
Plamonic, Core-satellite Nanostructures, Gold nanoparticles, Oligonucleotides
Disciplines
Materials Science and Engineering
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Jayakrishnan, Vineet kumar, "Amplification-Free Rapid Detection of Specific Oligonucleotides Through Formation of Core-Satellite Nanostructures on Solid Substrates" (2024). Material Science and Engineering Theses. 1.
https://mavmatrix.uta.edu/materialscieng_theses/1