Document Type

Honors Thesis

Abstract

Peripheral Artery Disease (PAD) is characterized by reduced blood flow, ischemic injury, and fibrosis, which collectively impair tissue function. Current treatment strategies are limited in their ability to target fibrotic tissue and restore normal function effectively. This study evaluates the therapeutic potential of drug-loaded nanoparticles to reduce fibrosis in ischemic tissue. Nanoparticles were synthesized and characterized for in vivo application. Tissue samples from experimental groups, including saline control, blank nanoparticles, free drug, and drug-loaded nanoparticles, were analyzed using Masson’s Trichrome staining to quantify collagen deposition. Histological images were processed using image analysis software to determine the percentage of collagen area. v Results demonstrated significant differences between treatment groups. Drug loaded nanoparticles showed the greatest reduction in collagen deposition compared to controls, indicating decreased fibrosis. Free drug treatment showed moderate effects, but was less effective than nanoparticle delivery. Statistical analysis confirmed a significant reduction in collagen content in the drug-loaded nanoparticle group. These findings suggest that nanoparticle-mediated drug delivery enhances therapeutic efficacy by improving drug localization and reducing fibrosis in PAD. This approach represents a promising strategy for improving treatment outcomes in ischemic diseases.

Disciplines

Biotechnology | Cell Biology | Other Analytical, Diagnostic and Therapeutic Techniques and Equipment

Publication Date

Spring 5-10-2026

Language

English

Faculty Mentor of Honors Project

Kytai Nguyen

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