Authors

Christina Koo

Document Type

Honors Thesis

Abstract

Bradyrhizobium japonicum is a nitrogen-fixing bacterium which forms a symbiotic relationship with leguminous soybean plants, converting atmospheric nitrogen into ammonia. The process of nodulation involves signaling components from the plant and activation of genes promote nodule formation. Soybean lectin’s current function in plants are unknown and may be a crucial component in nodulation. In previous studies, the copper tolerance protein, bll2211 (copB) was differentially expressed when exposed to soybean lectin. In this study, the role of environmental copper exposure was investigated to understand the role of soybean lectin in growth and nodulation of B. japonicum. We examined this by construction of a growth curve and performing a pouch experiment at various concentrations of copper for B. japonicum and its mutant ∆copB. Overall, there was less growth, nodule formation, and nitrogen-fixation in ∆copB. Thus, soybean lectin and copB may play a role in nodulation between B. japonicum and soybean plants.

Publication Date

5-1-2017

Language

English

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.