Document Type
Honors Thesis
Abstract
While the rate of surviving a myocardial infarction has increased in the past 60 years from 60% to 90%, the rate of Americans developing chronic heart disease such as heart failure, arrhythmias, and hypertensive heart disease has risen. This has led to the development of imaging technology such as Light Sheet Microscopy (LSM) and Light Field Microscopy (LFM) to study heart contractility and congenital heart disease via models such as cancerous organoids or Zebrafish. However, LSM and LFM results are highly dependent on the quality of the staining and cleared samples. This study looks to add to the growing field of optical tissue clearing (OTC) using the Binaree rapid clearing system based on non-organic electrophoresis clearing. The Binaree rapid clearing system demonstrated optimal results in microscale organoids in the passive method, which had required re-staining after clearing.
Disciplines
Bioimaging and Biomedical Optics | Biomedical Engineering and Bioengineering | Engineering | Molecular, Cellular, and Tissue Engineering
Publication Date
2025
Faculty Mentor of Honors Project
Juhyun Lee
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Lieu, Lauren D., "OPTIMAL TISSUE CLEARING OF GASTRIC CANCEROUS ORGANOIDS IMAGED VIA HIGH RESOLUTION LIGHT SHEET MICROSCOPY" (2025). 2025 Fall Honors Capstones Projects. 22.
https://mavmatrix.uta.edu/honors_fall2025/22
Included in
Bioimaging and Biomedical Optics Commons, Molecular, Cellular, and Tissue Engineering Commons