Eesha Khan

Document Type

Honors Thesis


DNA can be altered by introducing mutagens that can induce mutations and alter the genomes of organisms. The microcrustacean, Daphnia, was exposed to Proflavine, a mutagenic disinfectant, and the effects were seen by breeding the organisms and sequencing their genomes. Isolates of Daphnia were collected, and then solutions with the mutagen were prepared to determine the adequate concentration to utilize for exposure. After successive reproductions, the babies were monitored, then sent to sequencing to examine mutations present in the DNA. After many trials, the optimal concentration for Proflavine was determined to be 35 ug/mL, showing the ability of Daphnia to reproduce in subsequent generations in the presence of a mutagen. Sequenced genomes illustrated an increased rate of insertions and deletions (indels). On average, the indel mutation rate was 4.22 ×10-5 higher than the control for the Aromoose isolates and 1.01×10-4 for the Warner 5, signifying the presence of frameshift mutations. Research will continue being conducted to see the phenotypical effects in growing generations, thus creating a successful forward genetics screening method.

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