Applications Of Circular Dichroism (CD) Spectroscopy As An Educational Tool For Undergraduate Students And Monitoring Protein Conformational Changes Induced By Ligand Binding

Author

Catherine Ann Van Der Kar

Graduation Semester and Year

2012

Language

English

Document Type

Thesis

Degree Name

Master of Science in Chemistry

Department

Chemistry and Biochemistry

First Advisor

Brad Pierce

Abstract

The work reported here provides an overview on the applications of circular dichroism (CD) spectroscopy for the study of protein secondary structure. This project has two specific goals. First, to develop and validate an undergraduate experiment for CHEM 3175 (biophysical chemistry laboratory) to demonstrate the use of CD spectroscopy for the determination of protein secondary structure. In an extension of this work, the same principles of analysis were applied to the study and characterization of the protein-ligand interaction of MiaE (a non-heme iron hydroxylase) and its substrate, tRNA.

Disciplines

Chemistry | Physical Sciences and Mathematics

License

This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 International License.

Comments

Degree granted by The University of Texas at Arlington

Recommended Citation

Van Der Kar, Catherine Ann, "Applications Of Circular Dichroism (CD) Spectroscopy As An Educational Tool For Undergraduate Students And Monitoring Protein Conformational Changes Induced By Ligand Binding" (2012). Chemistry & Biochemistry Theses. 70.
https://mavmatrix.uta.edu/chemistry_theses/70