Chiral Recognition And Binding Constant Determination In Monovalent And Bivalent Systems Using Electrospray Ionization Mass Spectrometry

Author

Manishkumar Joshi

Graduation Semester and Year

2008

Language

English

Document Type

Thesis

Degree Name

Master of Science in Chemistry

Department

Chemistry and Biochemistry

First Advisor

Kevin A Schug

Abstract

Electrospray ionization mass spectrometry (ESI-MS) is a soft ionization method which is capable of transferring weakly bound host-guest noncovalent complexes from the solution phase to the gas phase. This capability makes ESI-MS an ideal tool for the quantification of binding affinities of different host-guest noncovalent complexes. There is a growing interest in using ESI-MS for chiral recognition studies. In thiswork ESI-MS was employed to investiga te the binding affinities for cinchona alkaloid chiral selectors binding N-blocked leucine enantiomers, as well as for macrocyclic antibiotics binding a variety of derivatized and underivatized amino acids. The effect of multivalency on binding affinity was also evaluated. This ESI-MS method is very promising for the binding studies of different host-guest systems. Merits of this method are speed, sensitivity and low sample consumption.

Disciplines

Chemistry | Physical Sciences and Mathematics

License

This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 International License.

Comments

Degree granted by The University of Texas at Arlington

Recommended Citation

Joshi, Manishkumar, "Chiral Recognition And Binding Constant Determination In Monovalent And Bivalent Systems Using Electrospray Ionization Mass Spectrometry" (2008). Chemistry & Biochemistry Theses. 45.
https://mavmatrix.uta.edu/chemistry_theses/45