Graduation Semester and Year
2014
Language
English
Document Type
Dissertation
Degree Name
Doctor of Philosophy in Chemistry
Department
Chemistry and Biochemistry
First Advisor
Kevin A Schug
Abstract
Continuous flow extractive desorption electrospray ionization (CF-EDESI) is a novel ambient ionization technique that was developed to help analyze samples from non-ESI-friendly solvents, manipulate protein charge states, and couple to a flowing sample solution stream. CF-EDESI is performed by introducing a sample solution through a hypodermic needle set orthogonal to an on-axis electrospray ionization source. Samples dissolved in non-ESI-friendly solvents such as hexanes, ethyl acetate etc. can be successfully detected in CF-EDESI which then makes it an attractive detection technique for normal phase separation. Additionally, introducing samples separated from electrospray has provided freedom to optimize both the sample solvents and spray solvents. Additives such as acetic acid, sulfolane, m-nitrobenzyl alcohol were used in spray solvents to manipulate the protein charge state, which also minimized the post column band broadening for separations when coupled with separation. Besides, ammonium acetate additive in spray solvents can greatly reduce the sodium adducts in lipid analysis from MTBE.
Disciplines
Chemistry | Physical Sciences and Mathematics
License
This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 International License.
Recommended Citation
Li, Li, "Fundamental And Applied Studies Of An Ambient Ionization Technique: Continuous Flow-extractive Desorption Electrospray Ionization - Mass Spectrometry" (2014). Chemistry & Biochemistry Dissertations. 67.
https://mavmatrix.uta.edu/chemistry_dissertations/67
Comments
Degree granted by The University of Texas at Arlington