ORCID Identifier(s)

0009-0002-0837-5627

Graduation Semester and Year

Summer 2025

Language

English

Document Type

Dissertation

Degree Name

Doctor of Philosophy in Biology

Department

Biology

First Advisor

Matthew Walsh

Second Advisor

Laura Mydlarz

Third Advisor

Shane Dubay

Fourth Advisor

Luke Frishkoff

Fifth Advisor

Alison Ravenscraft

Abstract

Ecological variables can induce the stress response in organisms. Yet, few studies have tested for evolutionary shifts in stress responses across ecological gradients. In these studies, we used Trinidadian killifish found in sites with predators (high predation, HP) and sites where they are the only fish present (killifish only, KO). HP and KO populations differ in a suite of behavioral and life-history traits, making the Trinidadian killifish an excellent model to examine evolutionary physiology. My dissertation addresses the knowledge gap by examining: (1) how baseline cortisol differs in wild-caught Trinidadian killifish found in HP and KO sites, (2) the influence of the direct and indirect effects of predation on the evolution of the stress response (cortisol levels) by rearing second-generation lab-born fish from sites with and without predators on contrasting food levels that reflect the known differences in growth between the communities, and (3) how divergent predation environments influence maternal effects on egg hormone levels and subsequent offspring performance. In chapter one, I demonstrate significant differences in baseline cortisol levels between HP and KO populations. I also observed variation across replicate rivers, potentially indicative of non-parallel divergence. In chapter two, I found that fish from sites with predators (HP) have evolved significantly lower baseline cortisol levels than fish that lack predators (KO). However, these differences were only apparent when fish were fed high food levels and disappeared under a low resource treatment that mimics growth in sites where predators are absent. Given the known differences in population density and resource availability between HP and KO sites, our results indicate that both the direct and indirect consequences of predation shape the evolution of the stress response. In chapter three, our findings revealed that KO females deposited higher levels of triglycerides and cortisol into their eggs, which was associated with reduced offspring growth. In contrast, HP females exhibited more moderate levels of deposition and evidence of a physiological buffering mechanism mitigating the negative impact of elevated cortisol. These results suggest that maternal transfer strategies are shaped by population-specific ecological histories, particularly the presence or absence of predators.

Keywords

Ecology, Evolution, Cortisol, Killifish, Maternal Effects

Disciplines

Behavior and Ethology | Comparative and Evolutionary Physiology | Evolution | Other Ecology and Evolutionary Biology

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