Graduation Semester and Year

2010

Language

English

Document Type

Dissertation

Degree Name

Doctor of Philosophy in Quantitative Biology

Department

Biology

First Advisor

Paul Chippindale

Abstract

In this study the main focus is to compare the methylation patterns of interspecific Xenopus F1 hybrids to the parental species using Methyl-Sensitive Amplification Polymorphisms (MSAPs). Genomic DNA was extracted from the liver and muscle tissues from a representative sample of F1 hybrids and the parental species (Xenopus laevis and X. muelleri). First, the MSAP markers were very effective in comparing the methylation patterns of hybrids and the parental species. Genetic analysis of 504 liver and 364 muscle MSAP markers revealed that these markers were highly polymorphic. Principal coordinate analysis showed four distinct clusters with the two parental species separate and the F1s in between with respect to levels and patterns of methylation. Most of the variance among clusters can be explained by within cluster (population) variation. There was more variation in the liver tissues. Furthermore, Nei's genetic distances revealed more dissimilarity between the clusters with the muscle tissues compared to liver. Also, despite the fact that at least 50% of the MSAP markers in the F1 hybrids are derived from the parental species, there is some loss that is biased to both parental markers (i.e. most of the parental fragments not detected in the F1 hybrids come from either the paternal or maternal species depending on the sex of the F1). Fisher's exact test indicates the proportion of the loss of diagnostic parental fragments in the F1 is significant for the muscle tissue. The hybrids also exhibited unique MSAP markers. The average fraction of methylated MSAP fragments generated in this study ranges from 70.1% to 78.8% (75.0 ± 4; mean± SD) and 67.0% to 73.8% (69.1 ± 3.2; mean± SD) for liver and muscle tissues respectively. Furthermore genome sizes of F1 hybrids were intermediate between those of the parents. Finally undermethylation was observed in liver tissues of male F1 hybrid.

Disciplines

Biology | Life Sciences

Comments

Degree granted by The University of Texas at Arlington

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