Graduation Semester and Year

2008

Language

English

Document Type

Thesis

Degree Name

Master of Science in Biomedical Engineering

Department

Bioengineering

First Advisor

Jian Yang

Abstract

Most of the works done on fluorescent polymers are focused on their potential applications in lighting industries. Much less interest has been paid for their potential application in the field of biology and biomedical engineering. Although multiple biological uses of green fluorescent proteins (GFPs) have been addressed by different groups, their potential for biomedical applications is hampered by their tendency to photobleach rapidly and their short fluorescence lifetime. Quantum dots have emerged as a potential substitute for those applications, but their cytotoxicity and lack of biodegradability restrict their use in a biological system. An ideal fluorescent material that could be used for both biological and biomedical purposes should be biocompatible and biodegradable. They should have strong emissive property and high photostability. The primary objective of this research was to develop biodegradable photolouminescent polymers (BPLPs) and nanoparticles using 1,8-Octanediol, Citric acid and different amino acids as the monomers. All of the twenty essential amino acids were used separately to create a family of BPLP. Owing to its better fluorescent properties, most of the experimental methods and discussion is based on BPLP with cysteine (BPLP-cys) in it. The ratio between the monomers was also changed in order to fine tune the color and the mechanical properties of BPLPs. Different polymers and nanoparticles thus formed were characterized using fourier transformed infrared spectrometer (FTIR), nuclear magnetic resonance (NMR), ultra violet-visible spectrophotometer and fluorospectrophotometer. The tensile strength of BPLP-cys was found to be in a range of 3.2±0.13 to 6.5±0.8 MPa and its modulus was as high as 7.02±1.40 MPa. BPLP-cys had a maximum elongation up to 240±36 %. The results from transmission electron microscopy (TEM) indicated a size that ranged between 50 to 450 nm for various concentrations of BPLP-cys. The polymers were shown to be cytocompatible through various cell culture studies. Lastly, BPLPs incorporated with other amino acids emitted light in various regions of visible spectrum. Hence, the family of BPLP can prove to be helpful in multi-color imaging applications.

Disciplines

Biomedical Engineering and Bioengineering | Engineering

Comments

Degree granted by The University of Texas at Arlington

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