Graduation Semester and Year

2012

Language

English

Document Type

Thesis

Degree Name

Master of Science in Biomedical Engineering

Department

Bioengineering

First Advisor

Hanli Liu

Abstract

Deoxyribonucleic Acid (DNA) molecules are informational molecules, encoding genetic information which is indispensable in the development and functioning of living organisms. DNA encodes for most proteins essential for all bodily functions and a few of which are known to be involved in the DNA Damage Response/ Repair mechanism. DNA damage recognition and repair is crucial and the cell has an innate mechanism of repairing it. Unrepaired DNA damage leads to genetic mutation or even cancers, which are sustained by further cell division and genetic multiplication. Other researchers have already confirmed the involvement of several proteins and histones like PARP and H2AX respectively in the DNA damage repair pathway. In this thesis, different proteins like Mixed Lineage Leukemia (MLL) and Homeobox protein Hox-B9 were screened to verify their role in DNA Damage Recognition/ Repair pathway. The response of these proteins upon Laser-induced DNA Damage was analyzed by corresponding anti-body staining as well as by fluorescence time-lapse imaging of GFP (Green Fluorescent Protein) tagged cells. The main aim is to evaluate role of MLLs and HOX proteins in the complex DNA Damage Repair pathway and its associated proteins.

Disciplines

Biomedical Engineering and Bioengineering | Engineering

Comments

Degree granted by The University of Texas at Arlington

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