Document Type

Article

Source Publication Title

Scientific Reports

First Page

1

Last Page

10

DOI

10.1038/srep30540

Abstract

Photobiomodulation, also known as low-level laser/light therapy (LLLT), refers to the use of red-tonear-infrared light to stimulate cellular functions for physiological or clinical benefits. The mechanism of LLLT is assumed to rely on photon absorption by cytochrome c oxidase (CCO), the terminal enzyme in the mitochondrial respiratory chain that catalyzes the reduction of oxygen for energy metabolism. In this study, we used broadband near-infrared spectroscopy (NIRS) to measure the LLLT-induced changes in CCO and hemoglobin concentrations in human forearms in vivo. Eleven healthy participants were administered with 1064-nm laser and placebo treatments on their right forearms. The spectroscopic data were analyzed and fitted with wavelength-dependent, modified Beer-Lambert Law. We found that LLLT induced significant increases of CCO concentration (?[CCO]) and oxygenated hemoglobin concentration (?[HbO]) on the treated site as the laser energy dose accumulated over time. A strong linear interplay between ?[CCO] and ?[HbO] was observed for the first time during LLLT, indicating a hemodynamic response of oxygen supply and blood volume closely coupled to the up-regulation of CCO induced by photobiomodulation. These results demonstrate the tremendous potential of broadband NIRS as a non-invasive, in vivo means to study mechanisms of photobiomodulation and perform treatment evaluations of LLLT

Publication Date

8-3-2016

Language

English

Comments

The authors acknowledge the support in part from the University of Texas BRAIN Initiative Seed Funding (#362718).

Department of Bioengineering, The University of Texas at Arlington

License

Creative Commons Attribution 3.0 License
This work is licensed under a Creative Commons Attribution 3.0 License.

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