Graduation Semester and Year
2011
Language
English
Document Type
Thesis
Degree Name
Master of Science in Materials Science and Engineering
Department
Materials Science and Engineering
First Advisor
Samarendra Mohanty
Abstract
Optical microscopy has fascinated biologists for probing and observing cellular structure while performing scientific research. While numerous wide-field and scanning microscopic techniques are being developed for probing cellular structure and function with high spatial and temporal resolution, mapping of complete biochemical, material and structural properties of the cell in three dimensions requires integration of multiple microscopic imaging techniques. In this thesis, conventional phase contrast microscopy and epifluorescence microscopy techniques were combined with other imaging methods such as digital holography microscopy (DHM) also known as quantitative phase microscopy, atomic force microscopy (AFM), near-field scanning optical microscopy (NSOM), total internal reflection fluorescence microscopy (TIRFM) and multiphoton microscopy (MPM). Introductory chapter will describe the importance of each technique and how the shortcoming of one technique is overcome by inclusion of other. Characterization of laser microbeam induced nanoscale manipulation was achieved using this multimodal imaging system.
Disciplines
Engineering | Materials Science and Engineering
License
This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 International License.
Recommended Citation
Ingle, Ninad, "Development Of Multimodal Imaging System Combined With Laser Microbeam For Nanoscale Manipulation And Characterization." (2011). Material Science and Engineering Theses. 43.
https://mavmatrix.uta.edu/materialscieng_theses/43
Comments
Degree granted by The University of Texas at Arlington