Document Type

Honors Thesis


Various tissue clearing methods have been made to optically clear tissue for high resolution imaging, but clearing whole organs is an arduous task due to limited penetration depth. To find a potential solution, 1 mm mouse organ slices were used to try four different variations of PEGASOS and BABB, organic solvent based clearing methods. Two of the variations were chosen to stain with antibodies and dyes, such as DLL4, to display vasculature. Axially swept-light sheet fluorescence microscopy was used to obtain 3D images of the kidneys. In addition, stained heart and lungs images were also acquired. Progress photos for clearing slices were observed under an inverted microscopy. The outcome was that PEGASOS is the most compatible clearing medium for staining while BABB was the best for viewing autofluorescence.

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