Chuo-Li Wang

Graduation Semester and Year

2006

Language

English

Document Type

Thesis

Degree Name

Master of Science in Mechanical Engineering

Department

Mechanical and Aerospace Engineering

First Advisor

Bumsoo Han

Abstract

Enhancement of cryoinjury by use of eutectic formation was investigated to improve the efficacy of cryosurgery. Although the enhancement of cryoinjury associated with eutectic crystallization of sodium chloride (NaCl) has been demonstrated, its advantage can be maximized with adjuvants which are biocompatible and have higher eutectic temperature than NaCl. In this study, the feasibility of glycine, which is less toxic and have higher eutectic temperature than NaCl, was tested. Two groups of MCF-7 human breast cancer cell were suspended in isotonic saline with glycine, then were frozen and thawed on a directional solidification stage in the same protocol except that eutectic crystallization was initiated in only one group. The results show that the post-thaw viability drops from 86% to 38.6% upon the initiation of eutectic crystallization at a high temperature (-8.5°C). The results also confirm that enhancement of direct cell injury during freezing can be achieved by eutectic crystallization.

Disciplines

Aerospace Engineering | Engineering | Mechanical Engineering

Comments

Degree granted by The University of Texas at Arlington

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