Graduation Semester and Year

2016

Language

English

Document Type

Thesis

Degree Name

Master of Science in Materials Science and Engineering

Department

Materials Science and Engineering

First Advisor

Pranesh B Aswath

Abstract

The use of bioactive material from bioceramic and composite components to support bone cell and tissue growth is an interesting area of medical field. Calcium phosphate ceramics are biocompatible and may develop interactions with human living bone tissues. Bioactive calcium phosphates, beta -tricalcium phosphate (β-TCP), have been intensively investigated as the cell scaffold for bone tissue engineering because it is well recognized that they are compatible to natural bone tissue and osteoconductive. In this research we will discuss two types of biomaterial with different kind of composition—(a) a cement type and (b)a scaffold type of biomaterial that can be applied to different kind of bone effect treatment. β-TCP is advantageous from the viewpoint of dissolution, but brittle compare to the other CPC. Its brittleness posses a limitation of their common clinical use. Current challenges include the engineering of materials that can match both the mechanical and biological property to enhance the recovery of bone failure. For the study, bioactive glass, gelatin, and laponite solution which that are is known forto their bioactive propertiesfeature will be usedapplied to create a bioactive scaffold with β-TCP. Groups of scaffold will be analysis in three direction approaches (1) Material characterization by using SEM, FTIR, XRD, EDS, goniometric machine…etc (2) In Vitro Study with cell culture, MTS assay, cell adhesion, raman spectra….etc (3) Development of TThree Dimensional Printed Scaffold. Findings suggest that better the optimum composition of b-TCP with 30% bioglass and eitherwith 2wt% laponite orand 2wt% Gelatin can lead to higher mechanical property and more enhanced formation of hydroxyapatite(HA). Cellular differentiation studies with xxxx cells in the presence involved addition of ascorbic acid (50 mg/L) also well performed demonstrated improved by observing the collagen fiber bundles formation compared to other compositions and the control coverslip. These results support the larger concept that beta tri-calcium phosphate with Bioglass and great binder like gelatin and laponite could control bone formation by inducing the osteogenic differentiation. With the understanding of the material characterization and pathology environment, using specialized three-dimensional (3D) printing technology can we created a porous structure to promote bone repair.

Keywords

Beta tri-calcium, Biomaterials, CPC, Biocement, Bioscaffold, Gelatin, Laponite

Disciplines

Engineering | Materials Science and Engineering

Comments

Degree granted by The University of Texas at Arlington

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