Graduation Semester and Year

2018

Language

English

Document Type

Thesis

Degree Name

Master of Science in Biomedical Engineering

Department

Bioengineering

First Advisor

Liping Tang

Second Advisor

Yi Hong

Third Advisor

He Dong

Abstract

There’re many existing imaging tools that composed of different imaging moieties to improve both resolution and sensitivity, however there’s not yet any imaging method that could be effectively utilized in both in vitro and in vivo study. To fill the gap, dual dyes-labeling fluorescent imaging nanoprobes was synthesized to simultaneous carry both visible fluorescent isothiocyanate (FITC) dye and near infra red (NIR) dye, 1,1′,3,3,3′,3′- hexamethylindotricarbocyanine iodide dye agent (HITC). For that, nanoparticles (NPs) was synthesized from 2-Hydroxyethyl methacrylate, styrene, acrylic acid and fluorescein-o-acrylate which contains visible FITC dye. The NPs were then labeled with HITC dye via physical entrapment to procedure HITC-FITC NPs. These nanoparticles have average size of 142.9nm±2.2, with high HITC loading efficiency (>90%). Our results confirm that both FITC and HITC dyes were successfully incorporated into NPs with high fluorescent intensity in both visible and NIR ranges. We have also observed possible static quenching effect caused by high concentrations of both dyes, whereas concentration of HITC increases reduce fluorescent intensity of FITC in NPs. By varying dye concentrations and ratios, we are able to fabricate HICT-FITC NPs with significant intensities in both NIR and visible ranges, with HITC/NPs weight ratio of 4X 10-4. Our results also confirmed that HICT-FITC NPs exhibits high photostability and colloid stability, as well as high aqueous-stability overtime. These particles also have low cytotoxicity with cancer cells, with upper limit concentration of 0.3mg/ml. With this particle concentration, different types of cells, esophageal, prostate, fibroblast and macrophage cell, were labeled and high resolution cell images was able to produce by microscope at visible light channel. Finally, particles labeled cancer cells was able to be monitored in animal model under near infra-red range imaging

Keywords

Nanoparticle, Near infrared dye, Fluorescein isothiocyanate, Styrene

Disciplines

Biomedical Engineering and Bioengineering | Engineering

Comments

Degree granted by The University of Texas at Arlington

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