Document Type
Thesis
Abstract
Sickle cell disease (SCD) results in erythrocyte deformation, contributing to vascular and bone complications. This study examined four-to-five-month-old SCD (n=6) and age-matched control (n=6) mice. Vascular function was assessed via wire myography (the descending aorta) and the isolated micro vessel technique (the femoral artery). For both vessels, endothelium-dependent and –independent vasodilation was determined to cumulative additions of acetylcholine (Ach, Log M) and DEA NONOate (DEA, Log M). The right femora were scanned via MicroCT (10µm) to determine trabecular bone microarchitecture (i.e., bone volume-to-total volume ratio, BV/TV [%], trabecular thickness [Tb.Th, µm], trabecular number [Tb.N, /mm], and trabecular separation [Tb.Sp, µm]). Cortical bone was assessed at the mid-shaft to determine cortical thickness (Ct.Th, µm) and ossified vessel volume (OsVV, µm) was determined in the marrow diaphysis. SCD mice exhibited lower body and spleen masses. Endotheliumdependent relaxation to ACh was significantly increased in SCD mice, while vasoconstriction responses were similar between groups. Trabecular BV/TV was reduced (5% vs. 12%, p
Disciplines
Cardiovascular Diseases | Cellular and Molecular Physiology | Exercise Science | Translational Medical Research
Publication Date
12-1-2024
Language
English
Faculty Mentor of Honors Project
Rhonda Prisby
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
McIntire, Jason, "Vascular Function and Trabecular Bone Phenotype In Sickle Cell Diseased Mice: Investigating The Interplay Between Vascular Dysfunction and Bone Health" (2024). 2024 Fall Honors Capstone Projects. 25.
https://mavmatrix.uta.edu/honors_fall2024/25
Included in
Cardiovascular Diseases Commons, Cellular and Molecular Physiology Commons, Exercise Science Commons, Translational Medical Research Commons