Document Type
Thesis
Abstract
Hyperbaric oxygen therapy (HBOT) can accelerate the regeneration of skeletal muscle after injury and improve overall recovery. Certain cells of the spleen are increased during muscle regeneration; however, the effect of HBOT on splenic cells during muscle regeneration is not well understood. In other experimental models, HBOT proliferates the proportion of splenic cells. In this study, eight male and nine female mice sustaining crush muscle injuries underwent HBOT at 1.5 atmospheres absolute for 60 min or normobaric hyperoxia (100% O2) for 60 min within 2-6 hours of injury for 4 days. The mice were then euthanized at 4- or 8-days post-crush, and the spleen was harvested. The proportion of splenic macrophages, monocytes, neutrophils, and dendritic cells were determined using flow cytometry. We see a heightened splenic cell response in female mice exposed to HBOT. Male mice exhibited a similar splenic cell response when exposed to either HBOT or 100% O2.
Disciplines
Biology | Medicine and Health Sciences
Publication Date
12-1-2024
Language
English
Faculty Mentor of Honors Project
Barbara St. Pierre Schneider
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Patel, Hetvi, "Effect of Hyperbaric Oxygen Therapy (HBOT) on the Proportion of Splenic Leukocytes during Skeletal Muscle Regeneration" (2024). 2024 Fall Honors Capstone Projects. 19.
https://mavmatrix.uta.edu/honors_fall2024/19