Document Type

Thesis

Abstract

Esophageal squamous cell carcinoma (ESCC) is a significant challenge due to its limited treatment options and poor prognosis. This study aimed to understand the irradiation induced cellular changes in ESCC cells to search for a better combination of radiotherapy and immunotherapy for ESCC patients through studying the effects of programmed death ligand-1 (PD-L1) and calreticulin (CRT), major cell immunogenicity markers. This research investigated the change in PD-L1 expression, CRT translocation, and how it impacted a cancer cell’s immune response. The human ESCC cell line, KYSE-150, was cultured and underwent irradiation. Images of the cells were taken using the Nikon A1R HD25 LSM confocal microscope and a statistical analysis was performed using Prism 5. In the cells, there was an increase in PD-L1 expression and CRT data is being processed. This cellular change indicates an increased immune response and contributes knowledge to expand treatment options for individuals affected by ESCC.

Publication Date

12-1-2024

Faculty Mentor of Honors Project

Zui Pan

License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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