Document Type
Thesis
Abstract
Esophageal squamous cell carcinoma (ESCC) is a significant challenge due to its limited treatment options and poor prognosis. This study aimed to understand the irradiation induced cellular changes in ESCC cells to search for a better combination of radiotherapy and immunotherapy for ESCC patients through studying the effects of programmed death ligand-1 (PD-L1) and calreticulin (CRT), major cell immunogenicity markers. This research investigated the change in PD-L1 expression, CRT translocation, and how it impacted a cancer cell’s immune response. The human ESCC cell line, KYSE-150, was cultured and underwent irradiation. Images of the cells were taken using the Nikon A1R HD25 LSM confocal microscope and a statistical analysis was performed using Prism 5. In the cells, there was an increase in PD-L1 expression and CRT data is being processed. This cellular change indicates an increased immune response and contributes knowledge to expand treatment options for individuals affected by ESCC.
Publication Date
12-1-2024
Faculty Mentor of Honors Project
Zui Pan
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Mirza, Ansah, "Irradiation Enhanced Immune Response in Esophageal Squamous Cell Carcinoma" (2024). 2024 Fall Honors Capstone Projects. 11.
https://mavmatrix.uta.edu/honors_fall2024/11