ORCID Identifier(s)

0000-0003-0803-1621

Graduation Semester and Year

2018

Language

English

Document Type

Thesis

Degree Name

Master of Science in Chemistry

Department

Chemistry and Biochemistry

First Advisor

Kevin A Schug

Second Advisor

Kevin A Schug

Abstract

Various supercharging agents, such as meta-nitrobenzyl alcohol, dimethyl sulfoxide, trifluoroethanol (TFE), and sulfolane were tested in different concentrations to study the effects of supercharging agents on the charge distributions of the high molecular weight intact proteins. The data were analyzed by triple quadrupole mass spectrometry. For most of the proteins, m-NBA and TFE worked more effectively than other supercharging agents, both to shift the charge state and increase intensity. The settings of different instrumental parameters were also studied. Both increases in source temperature and flow rate had a negative impact on average charge state observed. The goal of this work was to increase charge acquisition and ionization efficiency for proteins ranging from 66 kDa – 150 kDa, to allow subsequent optimization of multiple reaction monitoring (MRM) mode transitions for potential top-down quantitative analysis. The MRM transitions of six to eight different precursor ions of the proteins were optimized to demonstrate the feasibility of this approach and limits of detection were evaluated.

Keywords

Immunoglobulin G antibody, Trifluoroethanol, Albumin, Transferrin, Top-down, Electrospray ionization

Disciplines

Chemistry | Physical Sciences and Mathematics

Comments

Degree granted by The University of Texas at Arlington

Included in

Chemistry Commons

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