ORCID Identifier(s)

0000-0001-5000-5385

Graduation Semester and Year

2022

Language

English

Document Type

Thesis

Degree Name

Master of Science in Chemistry

Department

Chemistry and Biochemistry

First Advisor

Daniel W Armstrong

Second Advisor

Sherri A McFarland

Abstract

Given the importance of supercritical fluid chromatography, several works focused on the application use different SFC stationary phases. In this work, two new derivatized cyclofructan bonded phases with superficially porous particles are introduced in SFC. Sulfonic acid and benzoic acid groups were covalently linked to the cyclofructan molecule to make them polar and hydrophilic. These derivatives were then bonded to superficially porous particles for high-efficiency separations. This work also shows the effect of adding a small amount of water to the modified mobile phases in SFC. Traces of water in the co-solvent enhances the peak symmetry and efficiency of these new column chemistries. Moreover, we also illustrate the advantages of employing tetramethylammonium acetate for fast SFC. The new columns effectively separate polar and hydrophilic compounds, including acidic compounds, β blockers, and basic compounds of pharmaceutical and biological interest. HPLC using chiral stationary phases (CSPs) has proven to be the most widely employed method to separate enantiomers. This work presents HPLC method developments based on the existing CSPs for several chiral analytes. Chiral analytes were separated into six columns containing different chiral stationary phases (CSPs). The CSPs included with superficially porous particles bonded with two macrocyclic glycopeptides (Vancomycin, NicoShell), a cyclodextrin derivative (CD Shell-RSP: (hydroxypropyl-β-CD), a cyclofructan derivative (Isopropyl-derivatized CF6). And a cellulose derivative (IC: tris (3, 5-dichlorophenylcarbamate), and derivatized amylose (IG: tris (3-chloro-5 methyl phenyl carbamate), both bonded to fully porous particles were evaluated. The effects of various chiral selector structures, mobile phase compositions, and analyte structures have been studied.

Keywords

Supercritical fluid chromatography, Chiral ethanolamine substituted compounds

Disciplines

Chemistry | Physical Sciences and Mathematics

Comments

Degree granted by The University of Texas at Arlington

Included in

Chemistry Commons

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