ORCID Identifier(s)

0000-0002-2054-7279

Graduation Semester and Year

2017

Language

English

Document Type

Dissertation

Degree Name

Doctor of Philosophy in Chemistry

Department

Chemistry and Biochemistry

First Advisor

Saiful M Chowdhury

Abstract

Post-translational modification of proteins plays a significant role in the regulation of cellular processes. During the translation, process proteins undergo different modifications, which are known as post-translational modifications (PTMs). Due to their low abundances, PTM analysis presents several challenges; therefore, efficient and sensitive PTM detection methods are required. Arginine is an essential amino acid in a cell, which undergoes several kinds of PTMs. Finding these functional arginine residues in a protein is a challenging task. The task was successfully addressed, and the findings are not only reported herein, but an assessment was recently reported covering the new chemical labeling methods developed for identifying functional arginine residues of proteins by comparing two widely used arginine labeling reagents—1,2-cyclohexanedione (CHD) and phenylglyoxal (PG). This dissertation combined the author’s and her research team’s previous studies with bio-orthogonal chemistry and quantitative mass spectrometry-based proteomics to develop a new approach for the selective enrichment of reactive arginine residues in proteins in complex samples. The primary achievement was the development of a novel arginine-specific, azide-tagged CHD analog, which enables labeling of reactive arginine residues for further studies. For large-scale samples, the workflow from this labeling process is adaptable for gel-based pre-separation. This research established a promising strategy for the effective profiling of reactive arginine residues in large-scale studies. N-terminal modifications of proteins can interpret the functions and stability of proteins, affecting their expression, activation, or degradation. Moreover, selective enrichment of N-terminal peptides from a complex mixture is a difficult challenge in the proteomics field. Currently we are working with two novel reagents, which have innovative properties to enrich the N-terminal peptides and to generate marker ions from the N-term-labeled peptides during tandem mass spectrometry. These studies will significantly contribute to proteomics and to further research in the bio-analytical mass spectrometry field.

Keywords

Proteomics, Mass spectrometry

Disciplines

Chemistry | Physical Sciences and Mathematics

Comments

Degree granted by The University of Texas at Arlington

Included in

Chemistry Commons

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