Graduation Semester and Year

2016

Language

English

Document Type

Dissertation

Degree Name

Doctor of Philosophy in Chemistry

Department

Chemistry and Biochemistry

First Advisor

Daniel W Armstrong

Abstract

This dissertation focuses on two chromatographic techniques, gas chromatography (GC) and high performance liquid chromatography (HPLC). The goal of the GC work is to describe advances in separation methodologies focusing on the separation and quantitation of commercially related compounds (i.e. fatty acids, fatty amines, water, and ethanol). Four ionic liquid (IL) columns were evaluated for rapid analysis and improved resolution of long-chain methyl and ethyl esters of omega-3, omega-6, and additional positional isomeric and stereoisomeric blends of fatty acids found in fish oil, flaxseed oil, and potentially more complicated compositions. The potential for improved resolution of fatty acid esters is important for complex food and supplement applications, where different forms of fatty acids can be incorporated. Ionic liquid based capillary columns for GC were also used to separate trifluoroacetylated fatty amines focusing on the analysis of a commercial sample. Using an ionic liquid column, it was possible to separate linear primary fatty amines from C12 to C22 chain length in less than 25 min. Lastly, an ionic liquid GC method for the simultaneous quantitation of ethanol and water that is simple, accurate, precise, rapid, and cost-effective is demonstrated. Analysis of ethanol and water in consumer products is important in a variety of processes and often is mandated by regulating agencies. The goal of the remaining part of the dissertation is to demonstrate HPLC application for analyzing L- and D-amino acids in mouse tissues. The most complete characterization of brain and blood amino acid levels using a mouse model is performed. Hippocampus, cortex, and blood samples from mice were analyzed for L- and D-amino acid levels by a heart-cutting two-dimension liquid chromatography method. L- and D-amino acid levels are examined in terms of anomalies, trends and possible relevance to the limited existing data on mammalian D-amino acids.

Keywords

Gas chromatography, Liquid chromatography, D-amino acids, Fatty amines, Ionic liquid

Disciplines

Chemistry | Physical Sciences and Mathematics

Comments

Degree granted by The University of Texas at Arlington

Included in

Chemistry Commons

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